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Novel Fluorescence-Based Enzyme Complementation TechnologY
- The small 15-amino acid small subunit of the reporter enzyme is linked to the target of interest.
- The large subunit is added externally to the reaction together with the FRET substrate.
- The complemented enzyme cleaves the FRET substrate and releases the fluorescent signal.
- Cellular target engagement is measured as fluorescence signal generated by FRET substrate cleavage.
- The level of signal is proportional to stabilization of the target by the tested ligand.
- The assay can be used with conventional T-agg method as well as Real-Time Step-Gradient method (see white paper below).
Unprecedented Advantages
Target Agnostic
Target Agnostic
Target Agnostic
Leverage highly responsive system designed for your target. Choose from multiple target families - human or non-human.
DEL Enabled
Target Agnostic
Target Agnostic
Empower your drug discovery program with de novo discovery using DNA-Encoded Libraries (DELs) - all in the cell.
Sensitivity
Target Agnostic
Sensitivity
Enhance the breadth of your assays with highly sensitive cellular assays, from low nano-molar to high micro-molar levels.
Scalability
AI/ML Integration
Sensitivity
Scale your drug discovery effort for success. Use our sensitive cell systems for discovery and validation of novel drug candidates.
Real-Time
AI/ML Integration
AI/ML Integration
Upgrade your drug discovery data with real-time cell target engagement data. Reveal in-cell kinetics of drug-target engagement.
AI/ML Integration
AI/ML Integration
AI/ML Integration
Augment your drug discovery with the power of Artificial Intelligence. Tap into larger libraries and discover novel drug candidates.