The small 15 amino acid-long subunit of the reporter enzyme is linked to the target, while the large subunit is added externally to the reaction. The complemented enzyme cleaves the FRET substrate and releases the fluorescent signal. The level of signal is proportional to that of stabilization of the target by the tested ligand.
Sensitivity of signal
The level of fluorescence signal from Micro-Tag™ reporter cells is sensitive to the number of cells and level of expressed target protein.
Example of MTH1
MTH1 tested using Micro-Tag™ platform has temperature of aggregation (T-agg) of 55C. It engages S-Crizotinib with EC50 of 58nM
Unprecedented Advantages
Target Agnostic
Target Agnostic
Target Agnostic
Leverage highly responsive system designed for your target. Choose from multiple target families - human or non-human.
DEL Enabled
Target Agnostic
Target Agnostic
Empower your drug discovery program with de novo discovery using DNA-Encoded Libraries (DELs) - all in the cell.
Sensitivity
Target Agnostic
Sensitivity
Enhance the breadth of your assays with highly sensitive cellular assays, from low nano-molar to high micro-molar levels.
Scalability
AI/ML Integration
Sensitivity
Scale your drug discovery effort for success. Use our sensitive cell systems for discovery and validation of novel drug candidates.
Real-Time
AI/ML Integration
AI/ML Integration
Upgrade your drug discovery data with real-time cell target engagement data. Reveal in-cell kinetics of drug-target engagement.
AI/ML Integration
AI/ML Integration
AI/ML Integration
Augment your drug discovery with the power of Artificial Intelligence. Tap into larger libraries and discover novel drug candidates.