CORE APPLICATION AREAS
Cellular DEL Discovery
DNA-encoded library campaigns generate large numbers of candidate binders, but validating on-target engagement in cells remains a key challenge. MICRO-TAG® enables cellular validation of DEL hits to support confident prioritization.
Questions Addressed:
• Do DEL hits engage the target in cells?
• Which hits should be prioritized for synthesis?
• Are apparent binders biologically relevant?
Hit Validation & Optimization
Early hit lists often contain compounds that perform well in biochemical assays but fail to engage the target in cells. MICRO-TAG® enables direct measurement of cellular target engagement, helping teams distinguish true cellular hits from false positives.
Questions Addressed:
• Does this compound engage the target in cells?
• How does engagement compare across a series?
• Are observed phenotypes likely on-target?
Functional Target Engagement
Functional consequences of target engagement are often difficult to resolve using traditional assays. MICRO-TAG® provides a cellular approach to directly link compound binding with downstream biological effects.
Functional Responses :
• Signaling pathway activation
• Ternary complex formation
• Protein–protein interactions
